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Extraction and determination of ascorbate and dehydroascorbate from apoplastic fluid of stem of rooted and non-rooted cuttings in relation to the rhizogenesis.

机译:与生根有关的有根和无根插条茎的外生再生液中抗坏血酸和脱氢抗坏血酸的提取和测定。

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摘要

Starting from semihardwood shoots of peach rootstocks (Prunus cerasifera E.) cv. Mr.S.2/5, plum rootstocks (Prunus cerasifera × P. salicina) × (P. cerasifera × P. persica) cv. Ishtarà and olive tree (Olea europaea L.) cv. Frantoio, cuttings were collected and treated with 0 mgL-1 and 2500 mgL-1 of indolebutyric acid (IBA) by immersion the base of the cuttings. After 60 days of planting, the cultivars Mr.S. 2/5, Ishtarà and Frantoio presented a significative difference with relation to the rooting induction by application of the indolebutyric acid (27.5, 47.5 and 41.25 %, respectively), inducing a higher number of roots per cutting without at the same time significantly increasing the length of the roots themselves. The levels of ascorbate and dehydroascorbate in the rooted and non-rooted cuttings of the three cultivars were determined. These parameters varied in function of the consistency of the cuttings from each species, but showed a positive correlation between the increase of the rooting and the increase of the ascorbatein the reduced form.
机译:从桃砧木(Prunus cerasifera E.)cv的半硬木笋开始。 Mr.S.2 / 5,李子砧木(Prunus cerasifera×P. salicina)×(P. cerasifera×P. persica)cv。伊什塔拉(Ishtarà)和橄榄树(Olea europaea L.) Frantoio,收集插条,并通过将插条的基部浸入水中,用0 mgL-1和2500 mgL-1的吲哚丁酸(IBA)处理。种植60天后,栽培品种Mr.S. 2/5,Ishtarà和Frantoio在使用吲哚丁酸的生根诱导方面分别表现出显着差异(分别为27.5%,47.5%和41.25%),每次切割诱导更高的根数,而同时却没有显着增加根本身的长度。测定了三个品种的有根和无根插条中抗坏血酸和脱氢抗坏血酸的水平。这些参数随每种物种的插条的一致性而变化,但是在生根的增加与抗坏血酸在还原形式的增加之间显示出正相关。

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